Polyphenols from persimmon fruit attenuate acetaldehyde-induced DNA double-strand breaks by scavenging acetaldehyde

Acetaldehyde, a metabolic product of ethanol, induces DNA damage and genome instability. Accumulation of acetaldehyde due to alcohol consumption or aldehyde dehydrogenase (ALDH2) deficiency increases the risks of various types of cancers, including esophageal cancer. Although acetaldehyde chemically induces DNA adducts, the repair process of the lesions remains unclear. To investigate the mechanism of repair of acetaldehyde-induced DNA damage, we determined the repair pathway using siRNA knockdown and immunofluorescence assays of repair factors. Herein, we report that acetaldehyde induces DNA double-strand breaks (DSBs) in human U2OS cells and that both DSB repair pathways, non-homologous end-joining (NHEJ) and homology-directed repair (HDR), are required for the repair of acetaldehyde-induced DNA damage. Our findings suggest that acetaldehyde-induced DNA adducts are converted into DSBs and repaired via NHEJ or HDR in human cells. To reduce the risk of acetaldehyde-associated carcinogenesis, we investigated potential strategies of reducing acetaldehyde-induced DNA damage. We report that polyphenols extracted from persimmon fruits and epigallocatechin, a major component of persimmon polyphenols, attenuate acetaldehyde-induced DNA damage without affecting the repair kinetics. The data suggest that persimmon polyphenols suppress DSB formation by scavenging acetaldehyde. Persimmon polyphenols can potentially inhibit carcinogenesis following alcohol consumption.

In the present study, we investigated the underlying mechanisms of repair of acetaldehyde-induced DNA damage in human cells. We report that acetaldehyde induces DSBs, which are repaired by HR and NHEJ. Our findings suggest that acetaldehyde-induced DNA damage is recognised as DSBs and repaired in a manner similar to canonical DSBs in human cells. Since persimmon polyphenols are not toxic to humans and use acetaldehyde to generate bridges between molecules, they are candidates for the entrapment of acetaldehyde and its detoxification. Therefore, we focused on the potential application of persimmon polyphenols to minimise the risk of acetaldehyde-associated carcinogenesis. Using immunofluorescence assay, we examined the effect of persimmon polyphenols on acetaldehyde-induced DNA damage. We showed that polyphenols from persimmon fruit and EGC attenuated acetaldehyde-induced DNA damage. Persimmon polyphenols did not affect the repair kinetics of acetaldehyde-induced DNA damage. Therefore, we propose that persimmon polyphenols and EGC detoxify acetaldehyde by scavenging it. Our findings could be applied to reduce DNA damage and cancer risk associated with alcohol consumption.

DNA damage response pathway is activated by acetaldehyde treatment. Previous studies have
reported that acetaldehyde can induce DNA damage 2,3 . To determine the optimal conditions for detecting acetaldehyde-induced DNA damage, γH2AX focus formation and CHK1 phosphorylation at serine 345 (CHK1-pS345) were assessed as key events in DNA damage responses. CHK1 is phosphorylated at Ser 345 by the DNA damage response ATR kinase after DSB formation or the stalling replication fork, and is thus known as potential marker for monitoring the level of DNA damage. Asynchronous U2OS cells were treated with the indicated concentrations of acetaldehyde and then analysed for γH2AX focus formation (Fig. 1a). γH2AX foci were observed even in untreated cells (8.6%), resulting from spontaneous DNA damage or replication stress. The frequency of γH2AX-positive cells was not significantly altered after 0.1 mM acetaldehyde treatment (18.4%; Fig. 1a, b). A slight but statistically significant increase in the frequency of γH2AX-positive cells was observed when cells were treated with 1 mM acetaldehyde (24.8%). A marked increase in the frequency of γH2AX-positive cells was observed when cells were treated with 10 mM acetaldehyde (56.5%) compared to that in untreated cells (Fig. 1a,  b). Western blotting revealed that, consistent with the increased γH2AX focus formation, CHK1 phosphorylation signals were detected after 10 mM acetaldehyde treatment in HCT116 cells (Fig. 1c). Based on the increased www.nature.com/scientificreports/ γH2AX focus formation and CHK1 phosphorylation, we decided to use 10 mM acetaldehyde for subsequent experiments.
DPC formation by acetaldehyde treatment is less efficient than that by formaldehyde. To investigate the repair mechanisms of acetaldehyde-induced DNA damage, we first determined the types of DNA damage induced by acetaldehyde in vivo. Based on the similarity of its chemical structure to that of formaldehyde, acetaldehyde is believed to induce DPCs. A previous study suggested that acetaldehyde induces DPCs; however, there is still no direct evidence for the formation of DPCs by acetaldehyde 29 . Recently, a sensitive DPC detection assay (ARK assay) was developed 30 . ARK assays were used to assess the effects of acetaldehyde on DPC formation in vivo. As an experimental control, it was confirmed that 2.5 mM formaldehyde resulted in an eightfold increase in DPC formation compared to the untreated control, as previously reported 30 (Fig. 2a). Subsequently, the relative amount of DPC after treatment with 20 mM acetaldehyde was indistinguishable from that in the untreated U2OS cells (Fig. 2a). Furthermore, treatment with 100 mM acetaldehyde increased DPC formation significantly (2.4-fold), although the effect was clearly less than that after treatment with 2.5 mM formaldehyde (Fig. 2a). To verify the effect of acetaldehyde on DPC formation, the amount of DPC was determined using rapid DNA adduct recovery (RADAR) assays, another assay for DPC recovery 31 . In the RADAR assay, proteins covalently bound to genomic DNA are recovered from genomic DNA prepared under denaturing conditions. The amount of DPC was determined by measuring the quantity of the DPC-associated protein moieties. Similar to the results of the ARK assay, DPC formation did not increase following acetaldehyde treatment in U2OS cells (Fig. S1). The data suggest that acetaldehyde induces DPCs less efficiently than formaldehyde. Considering the increased γH2AX focus formation and CHK1 phosphorylation in the presence of 10 mM acetaldehyde, such observations suggested that acetaldehyde induces little or no DPCs in human cells.

Acetaldehyde induces DSBs in vivo.
A previous study reported that human BRCA1/2-deficient cells are hypersensitive to acetaldehyde, suggesting that HR factors are involved in the repair of acetaldehyde-induced DNA damage 22 . To examine the involvement of HR in the repair of such damage, we analysed the focus formation of RAD51, which marks sites of HDR, in U2OS cells. The frequency of RAD51 focus-positive cells was significantly increased after acetaldehyde treatment (45.8%) (Fig. 2b, c). RAD51 accumulates at stalled replication forks and DNA DSBs 19,32 , suggesting that acetaldehyde leads to replication fork stalling or DSB formation. Previous reports have demonstrated that γH2AX focus formation increases after acetaldehyde treatment 33 . γH2AX focus formation is caused by DSB formation or replication fork stalling without breaks (e.g. DNA adducts, DNA polymerase inhibitor, depletion of the dNTP pool) 34,35 . It is challenging to distinguish whether acetaldehyde induces DSBs or stalling of replication forks based on focus formation of RAD51 and γH2AX. To distinguish between the two possibilities, we analysed the focus formation of 53BP1, which is a molecular marker of DSBs, and functions in the NHEJ pathway. An increased frequency of 53BP1 focus-positive cells was observed after acetaldehyde treatment (47.6%) (Fig. 2d, e). The observations suggest that acetaldehyde induces DSBs in the genomic DNA in vivo. To confirm whether acetaldehyde induced DSBs, the number of DNA breaks on metaphase chromosomes was measured in HCT116 cells (Fig. 2f). DNA breaks in metaphase chromosomes were significantly increased after acetaldehyde treatment compared to those in untreated cells (3.9% in acetaldehydetreated cells and 1.2% in untreated cells) ( Fig. 2f and S2a). Consistent with a previous report 22,33 , our observations indicate that acetaldehyde can induce DSBs in vivo and suggest that HR and NHEJ are involved in the repair of acetaldehyde-induced DSBs. Considering acetaldehyde is a highly reactive molecule, our observations prompted us to test whether acetaldehyde chemically cleaves the DNA directly. To this end, we incubated linearised plasmids with acetaldehyde and analysed their fragmentation. After 3 h of incubation at 37 °C, no fragmented DNA was detected (Fig. S2b). Therefore, acetaldehyde did not directly induce DSBs.
HR and NHEJ are involved in the repair of acetaldehyde-induced DSBs. DSBs are mainly repaired using either HR or NHEJ. A few reports have shown that deficiencies in FA/HR factors cause DNA damage and reduce survival 5,22 . However, whether such factors are required for the repair of acetaldehyde-induced DNA damage and repair kinetics is poorly understood. Because RAD51 and 53BP1 accumulate in acetaldehydeinduced DSBs, we examined whether HR and NHEJ are required for the repair of acetaldehyde-induced DSBs.
To this end, we first analysed the DNA repair kinetics of acetaldehyde-induced DSBs in U2OS cells by monitoring the γH2AX foci as an indicator of DNA damage. After treatment with acetaldehyde for 24 h, 57.0% of cells were detected as being γH2AX-positive. The cells were then washed and released into acetaldehyde-free medium to monitor their recovery from acetaldehyde-induced DSBs. At 24, 48, and 72 h after release, γH2AX focus formation was assessed (Fig. 3a). At 24 h, a reduction in γH2AX-positive cells was observed, although the difference was not statistically significant ( Fig. 3b). At 48 and 72 h, the number of γH2AX-positive cells decreased to background levels (27.2% and 19.2%, respectively), indicating that acetaldehyde-induced DSBs were repaired within 72 h of release. To test whether HR and/or NHEJ are required for the repair of acetaldehyde-induced DSBs, we depleted RAD51C or XRCC4, key components of HR and NHEJ, respectively, from U2OS cells using specific siRNAs (Fig. 3c). The repair of acetaldehyde-induced DSBs was assessed by monitoring the formation of γH2AX foci (Fig. 3d). Forty-eight hours after release from acetaldehyde treatment, much higher levels of γH2AX-positive cells were observed in RAD51C-and XRCC4-depleted cells, compared to those in the control cells (67.2% in RAD51C-depleted cells, 59.1% in XRCC4-depleted cells, 37.8% in control cells) (Fig. 3e). The data indicate that the repair of acetaldehyde-induced DSBs was delayed in both HR-and NHEJ-deficient cells. Therefore, HR and NHEJ are required for the repair of acetaldehyde-induced DSBs. www.nature.com/scientificreports/ www.nature.com/scientificreports/ www.nature.com/scientificreports/ Persimmon fruit extract attenuates acetaldehyde-induced DSB formation. Acetaldehyde induces DNA damage and genome instability, which promotes carcinogenesis 3 . Indeed, acetaldehyde accumulation during alcohol consumption increases the risk of cancer in humans and mice 10,11 . Compounds that detoxify acetaldehyde may be useful for inhibiting carcinogenesis. To identify such detoxifying compounds, we focused on persimmon fruit. Traditionally, acetaldehyde, which is produced from ethanol, has been used to remove astringency in persimmon fruit 23 . During the removal process, acetaldehyde efficiently reacts with polyphenols from the fruit, leading to the formation of insoluble and non-astringent polyphenol aggregates through a non-enzymatic process 23,25 . Since insoluble complexes composed of acetaldehyde and polyphenols are unable to penetrate cell membranes, we considered the possibility that polyphenols from persimmon fruit might detoxify acetaldehyde.
To assess the effect of persimmon polyphenols on acetaldehyde-induced DNA damage, we simultaneously added acetaldehyde and the polyphenol fraction prepared from persimmon fruit to the media and monitored γH2AX focus formation (Fig. 4a). After acetaldehyde treatment in the absence of persimmon polyphenols, 60.7% of the cells were determined as being γH2AX focus-positive in U2OS cells. In the presence of 1 µg/ml and 0.1 µg/ml persimmon polyphenol fraction, the frequency of γH2AX focus-positive cells was reduced to 33.9% and 53.9%, respectively (Fig. 4a). To verify the effect of persimmon polyphenols on acetaldehyde-induced DNA damage, CHK1 phosphorylation and focus formation of RAD51 and 53BP1 were assessed. CHK1 phosphorylation was reduced by the addition of 1 µg/ml and 10 µg/ml persimmon polyphenol fractions to HCT116 cells (Fig. 4b). Consistent with the reduction in γH2AX focus formation, the frequency of 53BP1-and RAD51-focus positive cells was significantly decreased in the presence of the 1 µg/ml polyphenol fraction in U2OS cells (Fig. 4c,  d). Intriguingly, although the simultaneous addition of acetaldehyde and the 0.1 µg/ml persimmon polyphenol fraction had minimal or no effect on γH2AX, RAD51, and 53BP1 focus formation, a significant decrease was observed when the same concentration of persimmon polyphenol fraction was pre-incubated with acetaldehyde ( Fig. 4a, c, d). Such observations suggest that persimmon polyphenols attenuate the DNA damaging potential of acetaldehyde. Alternatively, persimmon polyphenols may facilitate repair of acetaldehyde-induced DNA damage. To exclude the latter possibility, we assessed their effect on repair by adding polyphenols after inducing DNA damage in U2OS cells (Fig. 4e). At 24 and 48 h after the removal of acetaldehyde with or without persimmon polyphenols, the frequency of γH2AX-positive cells was not altered by the addition of the persimmon polyphenol fraction (Fig. 4f). This observation suggests that persimmon polyphenols detoxify acetaldehyde by attenuating its DNA-damaging potential rather than by facilitating DNA repair, although we cannot exclude the possibility that polyphenols themselves affect cellular responses, such as gene expression or post-translational modifications.
EGC is an acetaldehyde scavenger and attenuates acetaldehyde-induced DNA damage. Persimmon polyphenols (tannins) are complex and heterogeneous forms of polymerised polyphenols. The reduced DNA damage after treatment with the persimmon polyphenol fraction prompted us to test whether the components of persimmon polyphenols could detoxify acetaldehyde. A previous study has reported that persimmon polyphenols consist of epicatechin (EC), epicatechin gallate (ECg), epigallocatechin (EGC) and epigallocatechin gallate (EGCg) 24 . Among these, the major component is EGC 23,24 . Therefore, we examined the effects of EGC on acetaldehyde-induced DNA damage in U2OS cells. To this end, we monitored γH2AX focus formation in the presence or absence of EGC. In the presence of 0.1 mM EGC, the frequency of γH2AX focus-positive cells decreased to 40.9%, whereas 70.2% were observed after acetaldehyde treatment only (Fig. 5a, b). Similar to the persimmon polyphenol fraction, pre-incubation with acetaldehyde and 0.01 mM EGC efficiently attenuated acetaldehyde-induced DNA damage (Fig. 5a, b). Consistent with the reduced γH2AX focus formation, CHK1 phosphorylation was also decreased by EGC treatment in HCT116 cells (Fig. 5c). The observations suggest that persimmon polyphenols can detoxify acetaldehyde.
A previous study reported that acetaldehyde bridges catechins and produces catechin polymers, raising the possibility that catechins, including EGC, scavenge acetaldehyde 23,26 . In that case, acetaldehyde can be used for the polymerisation of catechins, and the concentration of acetaldehyde can be reduced. To examine this possibility, we measured acetaldehyde concentration after incubation with EGC using an acetaldehyde detection kit. Incubation with 1 mM, 5 mM, and 10 mM EGC decreased acetaldehyde present in the reaction by 23.5%, 23.8%, and 55.1%, respectively, relative to that in the absence of EGC (Fig. 5d). The observations suggest that EGC scavenges acetaldehyde and attenuates acetaldehyde-induced DNA damage.

Discussion
Several studies have reported that acetaldehyde induces DNA damage 2,3 . However, the type of DNA damage induced by acetaldehyde and its cellular responses remain largely unclear. Here, using immunofluorescence assay of DNA repair factors, we found that acetaldehyde induced DSBs, which are repaired by DSB repair pathways, HDR and NHEJ (Fig. 6). Our observations suggest that acetaldehyde-induced DNA damage is recognised as a DSB in vivo. In addition, because acetaldehyde is generated during the metabolic processing of ethanol, it is important to develop a method to reduce the DNA-damaging potential of acetaldehyde to inhibit carcinogenesis. Based on the chemical reaction between acetaldehyde and polyphenols, we focused on polyphenols and established that polyphenols from persimmon fruit detoxified acetaldehyde by reducing DNA damage. The result implies that the intake of persimmon fruit reduces the risk of acetaldehyde-associated carcinogenesis. Overall, we determined the repair pathway of acetaldehyde-induced DSBs and suggested the potential use of persimmon polyphenols in detoxification.
Previous studies have shown that acetaldehyde chemically modifies nucleotides, such as DPCs, ICLs, and DNA adducts 2,3,36 . However, the type of DNA damage induced by acetaldehyde in the cells remains controversial. Here, we found that DSBs are induced by acetaldehyde and recognised by the HDR machinery protein RAD51 www.nature.com/scientificreports/ and NHEJ protein 53BP1 (Fig. 2b-e). Previous studies have suggested that acetaldehyde induces DPCs 29 . Using sensitive DPC detection assays, we found that acetaldehyde induced little or no DPCs in genomic DNA (Fig. 2a, S1). Thus, it seems that DPCs are not a major cause of genome instability induced by acetaldehyde and that the DPC repair pathway is not necessary to maintain genome stability after acetaldehyde treatment. In terms of ICLs, it has been reported that acetaldehyde induces ICLs and that acetaldehyde-induced ICLs are repaired by the FA pathway or another replication fork convergence-dependent pathway in a X. laevis egg extract system 16 .
In the general mechanism of FA pathway-dependent ICL repair, replication forks encounter ICLs induced by DNA crosslinkers, such as cisplatin, and XPF-ERCC1, a structure-specific endonuclease, is recruited to create nucleolytic incisions that generate DSBs 13,14 . Such DSBs are repaired by HDR 13,14 . In the present study, we were unable to provide direct evidence that acetaldehyde induces ICLs in the human genomic DNA. However, our finding that acetaldehyde treatment increased RAD51 focus formation and the requirement of RAD51C for repair implied that acetaldehyde-induced ICLs, which are converted to DSBs, are repaired in a manner similar to the repair of canonical ICLs during the S phase of the cell cycle. Such observations are consistent with the requirement of BRCA1/2 for survival after acetaldehyde treatment 22 . Furthermore, we showed the cytological kinetics of the repair (Fig. 3b). Recently, several replication-dependent pathways repair ICLs 16,37 . Although we demonstrated the kinetics of the repair, it is difficult to directly detect acetaldehyde-induced ICLs in vivo and investigate the relationships among these repair pathways in human cells. In future, techniques for detecting or visualising acetaldehyde-induced ICL/DSB should be developed. In contrast to replication-dependent repair, NHEJ repairs DSBs in a replication-independent manner and primarily functions in the G1 phase of the cell cycle 21 . Our findings that acetaldehyde induced 53BP1 focus formation (Fig. 2d, e) and that XRCC4 is required for the repair of acetaldehyde-induced DSBs (Fig. 3e) imply that, in the G1 phase, DSBs are induced by acetaldehyde in a replication-independent manner and repaired by the NHEJ pathway. Unexpectedly, direct cleavage of DNA strands was not observed after the incubation of DNA molecules with acetaldehyde (Fig. S2b). Thus, acetaldehyde treatment indirectly cleaved DNA strands in vivo. Because acetaldehyde potentially gives rise to bulky adducts, such adducts can be directly recognised and cleaved by specific endonucleases during the repair process in G1 phase. Acetaldehyde forms guanine adducts, including covalent guanine dimers and ICLs 2,3,36 . Similar to pyrimidine dimers, covalent guanine dimers may be recognised and cleaved by the nucleotide excision repair pathway in a replication-independent manner. Alternatively, guanine-rich sequences may form four-stranded secondary structures, G-quadruplexes (G4), and acetaldehyde may stabilise G4 structures. A recent study showed that stabilised G4 structures are recognised in a transcription-dependent manner and converted to DSBs by topoisomerase 2 activity 38 , which implies that acetaldehyde-induced guanine adducts are recognised by a similar mechanism. Our findings suggest that acetaldehyde induces DSBs in replication-dependent and replication-independent manners. In contrast to the wellcharacterised replication-dependent DSB formation, little is known about the replication-independent pathways. Therefore, the replication-independent DSB formation pathway mechanism requires further investigation.
DSBs cause genome instability and promote carcinogenesis. Indeed, mutations involving HDR or NHEJ genes, such as Brca1, Brca2, Mre11, Nbs1, and Lig4, predispose patients to several types of cancers 19,39 . Previous studies have reported that alcohol intake is associated with an increased risk of several types of cancer 10,11 . Considering acetaldehyde is a metabolic intermediate of ethanol that induces DNA damage, acetaldehyde-induced DNA damage could be a cause of the observed increase in cancer risk due to alcohol consumption. It is important to detoxify acetaldehyde to inhibit alcohol-induced carcinogenesis provoked by alcohol consumption. Based on the reactivity of persimmon polyphenols with acetaldehyde ( Fig. 6) 23,25 , we investigated whether persimmon  www.nature.com/scientificreports/ polyphenols could detoxify acetaldehyde. Intriguingly, the persimmon polyphenol fraction and EGC, a major component of persimmon polyphenols, attenuated acetaldehyde-induced DNA damage (Figs. 4 and 5). The effects of the persimmon polyphenol fraction and EGC include (1) pre-incubation of persimmon polyphenol or EGC with acetaldehyde effectively reduced DNA damage; (2) incubation of acetaldehyde with EGC decreased acetaldehyde concentrations 26 ; and (3) repair kinetics of acetaldehyde-induced DSBs were not altered by the addition of the persimmon polyphenol fraction. Therefore, persimmon polyphenols attenuate acetaldehyde-induced DNA damage by scavenging acetaldehyde rather than facilitating DNA repair (Fig. 6). Such data suggest that the detoxification of acetaldehyde by persimmon polyphenols may reduce the risk of cancer caused by alcohol consumption. Acetaldehyde forms bridges between catechins 23 . Therefore, similar reactions generate covalent bonds between the persimmon polyphenols. Since the structures of persimmon polyphenols are complex forms of polymerised polyphenols, persimmon polyphenols have numerous contact surfaces between molecules, which makes the reaction highly efficient. Following alcohol consumption, more than 30% of East Asians and 8% of the world population exhibit an alcohol flushing response due to inherited deficiency of ALDH2, which catalyses the reaction from acetaldehyde www.nature.com/scientificreports/ to acetic acid 12 . In addition to the alcohol flushing response, ALDH2 deficiency increases the risk of alcoholrelated cancer 10,11 . Based on the reduction in acetaldehyde-induced DNA damage by persimmon polyphenols, we propose that persimmon intake can potentially minimise the flushing response and future risk of cancer caused by alcohol consumption. In the present study, the effects of persimmon polyphenols on tissues and the body after ethanol intake were not tested. Experiments using animals with or without ALDH2 mutations are required to verify acetaldehyde detoxification by polyphenols in tissues and bodies.

Methods
Cell culture. All cell lines used in the present study were obtained from the American Type Culture Collection (ATCC). U2OS cells were used for immunofluorescence analysis because they express wild-type p53 and are suitable for use in analyses DNA damage responses and apoptosis. HCT116 cells were used for metaphase spread and western blotting analyses because they have a stable karyotype and lower level of spontaneous DNA damage. Both cell lines were immortalised. Cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 1% antibiotic-antimycotic mixed solution (Nacalai, 02892-54). All experiments were performed in accordance with institutional guidelines and regulations approved by the Kindai University Genetic Recombination Experiment and Biosafety Committee. www.nature.com/scientificreports/ Antibodies.  (Millipore 05-636), anti-RAD51 (Millipore ABE257), and anti-53BP1 (Novus Biologicals NB100-304) antibodies were used for the immunofluorescence analysis. Anti-CHK1-pS345 (Cell Signalling Technology 2348), anti-XRCC4 40 , anti-RAD51C (Novus Biologicals NB100-177), and anti-tubulin (Sigma, T4026) antibodies were used for the western blot analysis.
Western blot. HCT116 and U2OS cells were resuspended in benzonase buffer (20 mM Tris-HCl (pH 7.5), 40 mM NaCl, 2 mM MgCl 2 , 0.5% NP-40, 50 U/ml benzonase, protease inhibitor cocktail, PhosSTOP (Roche, 4906845001), and incubated on ice for 10 min. NaCl (5 M) was then added to the samples at a final concentration of 450 mM. The samples were rotated at 4 °C for 30 min and then centrifuged at 13,000 rpm for 10 min. The supernatants were separated using 15% SuperSep Ace (Wako, 193-14991) and transferred onto PVDF membranes. The membranes were blocked with 5% skim milk and subsequently incubated with primary antibodies at 4 °C overnight. After overnight incubation, the membranes were incubated with AP-conjugated secondary antibodies and treated with a BCIP-NBT alkaline phosphatase solution (Nacalai, 03937-60) for detection. Images were cropped and processed using Photoshop 2020 (Adobe, USA).
Immunofluorescence staining. U2OS cells cultured on coverslips were permeabilised with CSK buffer (10 mM PIPES pH 6.8, 100 mM NaCl, 300 mM sucrose, 3 mM MgCl 2 , 1 mM EDTA, 0.5% Triton-X100, protease inhibitor cocktail, PhosSTOP) for 5 min on ice. After permeabilisation, cells were fixed in 2% paraformaldehyde at room temperature (25 °C) for 15 min, then blocked in blocking buffer (PBS containing 10% goat serum, 5% BSA, 0.1% Triton X-100) at room temperature for 30 min. After blocking, the coverslips were incubated in blocking buffer containing the primary antibody at 4 °C overnight. Coverslips were washed in PBS containing 0.1% Triton X-100 three times and incubated with secondary antibodies at room temperature for 1 h. The cells were stained with DAPI and mounted using Vectashield (Vector Laboratories, H-1000).
ARK assay. The ARK assays were performed as described previously 30  www.nature.com/scientificreports/ Cleavage of plasmid by acetaldehyde. Plasmid pBluescript SK + was digested with EcoRV and precipitated using standard ethanol precipitation methods. The digested plasmid DNA was then resuspended in distilled water. Five hundred microliters of digested plasmid was incubated with the indicated concentrations of acetaldehyde at 37 °C for 3 h. Samples were separated on 0.7% agarose gels and stained with ethidium bromide.

Preparation of persimmon fruit polyphenols and EGC.
Persimmon fruit polyphenols were prepared as previously described 42 . Immature persimmon fruit cultivated in Japan was treated with ethanol to remove astringency. After homogenisation using a juice extractor, the homogenates were separated into four layers by centrifugation (1630×g, 15 min). The second layer from the bottom, containing tannin cells, was collected and lyophilised. The resulting powder was extracted with 100 volumes of water at 121 °C for 15 min and lyophilised again. Lyophilised polyphenols were dissolved in dimethyl sulfoxide (DMSO). Polyphenols from persimmon fruits used in this study consisted of 70% polyphenol (93% of which were proanthocyanidins) and 20% carbohydrate, as analysed using the Folin-Ciocalteu (using vanillin-HCl) and phenol-H 2 SO 4 methods, respectively. Using a combination of thiolysis and liquid chromatography-mass spectrometry analysis 43 , the structure of persimmon polyphenols from the variety Tonewase, as major compounds of Kaki-tannin (Nara-type), have been reported to consist of EC, ECg, EGC, and EGCg at a ratio of 1:1:2:2 (50% galloylated) 44 . The catechins were polymerised with a B-type linkage, and their average molecular weight was calculated to be approximately 8000 Da, in a previous study 45 . ECg was determined to be the terminal unit. EGC (Wako, 059-08951) purified from green tea, was dissolved in DMSO and used at the indicated concentrations.
Acetaldehyde concentration measurement. Acetaldehyde was incubated at 37 °C for 9 h in the presence or absence of EGC. An enzyme-linked acetaldehyde assay kit (BioAssay Systems, EFAC-100) was used to detect acetaldehyde in samples. Briefly, diluted samples were mixed with assay buffer, probe, NAD, and enzymes. After 30 min of incubation, the samples were analysed using an Infinite F200 Pro (TECAN, Switzerland) instrument. www.nature.com/scientificreports/